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A) 多数情况下是因为样品的数量太少。 细菌量一般在1亿个细菌左右(1ml 1OD600);B) 细胞未充分裂解; C) 增加溶菌酶的量; D) 延长裂解时间;E) 可适当减少洗脱液的体积。
A) Typically it was due to starting material too low, using 1 ml of 1 OD600; B) Bacterial cells were not completely lysed; C) Increase lysozyme; D) Increase lysis time; E) Reduce the volume of the elution buffer.