如何提高琼脂糖胶DNA回收量？How to improve DNA recovery from agarose gel?

如何提高琼脂糖胶DNA回收量？





A) DNA起始量较少; B) 短波长UV照射时间太长; C) 溶胶时间不够; D) 对小于200bp DNA片段，可增加与磁珠的接触时间或适当减少溶胶液的体积。

A) Not enough DNA to start with; B) UV radiation too long; C) Gel was not completely dissolved.

D) If DNA fragment <200 bp, you need add longer time to incubate with magnetic beads, or reduce GE10 volume, add GE20.